Identification of Specific Variations in a Non-Motile Strain of Cyanobacterium Synechocystis sp. PCC 6803 Originated from ATCC 27184 by Whole Genome Resequencing

Qinglong Ding,Gu Chen,Dong Wei,Yuling Wang

doi:10.3390/ijms161024081

Qinglong Ding, Gu Chen + Show 2 more

Open Access

https://doi.org/10.3390/ijms161024081

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Abstract

Cyanobacterium Synechocystis sp. PCC 6803 is a widely used model organism in basic research and biofuel biotechnology application. Here, we report the genomic sequence of chromosome and seven plasmids of a glucose-tolerant, non-motile strain originated from ATCC 27184, GT-G, in use at Guangzhou. Through high-throughput genome re-sequencing and verification by Sanger sequencing, eight novel variants were identified in its chromosome and plasmids. The eight novel variants, especially the five non-silent mutations might have interesting effects on the phenotype of GT-G strains, for example the truncated Sll1895 and Slr0322 protein. These resequencing data provide background information for further research and application based on the GT-G strain and also provide evidence to study the evolution and divergence of Synechocystis 6803 globally.

Highlights

As the first sequenced photosynthetic organism and with high transformation competency, the freshwater cyanobacteria Synechocystis sp
PCC 6803 was isolated from freshwater in California [6] and deposited in the Pasteur Culture Collection as PCC 6803 strain and in the American Type Culture Collection as ATCC 27184 strain
The glucose tolerant strain originated from ATCC 27184 through routine laboratory culture conditions in our lab was designated GT-G (Guangzhou) and subjected to genomic re-sequencing

Summary

Introduction

As the first sequenced photosynthetic organism and with high transformation competency, the freshwater cyanobacteria Synechocystis sp. PCC 6803 was one of the most widely used model. 2015, 16 organisms for the research in photosynthesis and stress response, as well as for the biotechnological application of biofuel production [1,2,3,4,5]. The original Berkeley strain of Synechocystis sp. PCC 6803 was isolated from freshwater in California [6] and deposited in the Pasteur Culture Collection as PCC 6803 strain and in the American Type Culture Collection as ATCC 27184 strain

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