Abstract
ABSTRACTIntroductionInvasion of microorganisms and their multiplication in root canals (RCs) results in endodontic infections of primary teeth. Acute and chronic inflammation may be present in the periapical area and are based on the amount and virulence of microorganisms, especially anaerobic bacteria present in the RC. To identify microorganisms very precisely in endodontic infections, polymerase chain reaction (PCR) is used.AimThe aim of the present study is to identify the specific anaerobic bacteria like Porphyromonas gingivalis, Prevotella intermedia, and Actinomyces naeslundii in the RCs of primary teeth using real-time PCR.MethodologyFifteen subjects aged 3–8 years who had endodontic infections in primary molars were selected. The cases who had been selected did not receive any endodontic treatment and antibiotics within 3 months, and children with systemic diseases were not included.Sample collectionSamples were taken by placing absorbent paper points into the largest canals of maxillary and mandibular molars for 60 seconds and are then transferred to a sterile Eppendorf tube with tris-hydochloride EDTA (TE) buffer. The samples were stored at −80°C. All samples were subjected to PCR analysis.ResultThe specific anaerobes detected in the samples were A. naeslundii (93.3%), Prevotella intermedia (53.3%), and Porphyromonas gingivalis (13.3%).ConclusionThe results suggested a high bacterial diversity in the RCs of infected primary teeth.How to cite this articleThimmegowda U, Thomas J, et al. Identification of Specific Anaerobic Bacteria in Endodontic Infections of Primary Teeth—A PCR Study. Int J Clin Pediatr Dent 2019;12(1):1–4.
Highlights
Invasion of microorganisms and their multiplication in root canals (RCs) results in endodontic infections of primary teeth
The purpose of this study is to detect and identify the specific microorganisms like Porphyromonas gingivalis,Prevotella intermedia, and Actinomyces naeslundiiin the RCs of human deciduous molars with endodontic infections using real-time polymerase chain reaction (PCR)
Different methods involved in the detection and identification of microorganisms in endodontic microbiota are DNA–DNA hybridization, denaturing gradient gel electrophoresis fingerprint, polymerase chain reaction (PCR), or DNA sequencing techniques.[2]The present study focuses on the detection of anaerobic microorganism associated with endodontic infection of deciduous dentition using molecular genetics, polymerase chain reaction which is more specific and sensitive in detecting microorganisms
Summary
Invasion of microorganisms and their multiplication in root canals (RCs) results in endodontic infections of primary teeth. To identify microorganisms very precisely in endodontic infections, polymerase chain reaction (PCR) is used. Aim:The aim of the present study is to identify the specific anaerobic bacteria like Porphyromonas gingivalis,Prevotella intermedia,and Actinomyces naeslundiiin the RCs of primary teeth using real-time PCR. Result: The specific anaerobes detected in the samples were A. naeslundii(93.3%), Prevotella intermedia(53.3%), and Porphyromonas gingivalis(13.3%). The species most frequently found in the pulp chamber of primary teeth are Porphyromonas gingivalis(73.3%), Prevotella intermedia(6.7%), Porphyromonas nigrescens(86.7%), and Fusobacterium alocis(73.3%). Found microbial species in RCs of primary teeth were Porphyromonas gingivalis(100%), Prevotella intermedia(6.7%), Actinomycosis naeslundii(7.1%), Porphyromonas nigrescens(93.3%), and Treponema forsythia(26.7%).[1]. Molecular genetics methods have been used recently in endodontic infections, for the identification of microorganisms. PCR assays are sensitive and identification of microbes or strains that are even impossible to culture can be identified very precisely.[2]
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