Abstract
The RAPD‐PCR technique was employed to identify three endemic Spanish species of Barbus: Barbus bocagei, B. graellsii and B. sclateri, that present very similar morphologies. Using seven primers, six diagnostic bands were found in B. bocagei, 11 in B. graellsii and nine in B. sclateri. Cluster analysis of the genetic similarity values obtained from RAPD data indicated that the species B. bocagei and B. graellsii are more related to each other than to B. sclateri.
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