Abstract
The application of molecular genetics to the identification of fishes is an appropriate technology for quality control and commercial tracking. We have applied a DNA-based methodology to identify the exact species of Argentinean hake included in commercial products. The first test consisted in the PCR amplification of a 122 bp fragment from the cytochrome bgene to assess the presence of DNA from hakes. This test being positive, it was followed by a second test consisting of the amplification and digestion of a PCR fragment of 464 bp from the cytochrome bgene, to determine the identity of the commercialized species. Since a single restriction enzyme provided full diagnosis of the southern Atlantic hake species present in the samples, this methodology would be of great help in traceability across the food-chain that comprises (1) the authentication of Argentinean hake products, (2) the control of fraud to consumers, (3) the industrial advice regarding the labeling of commercial products, and (4) the fishery forensics of Argentinean hakes.
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