Abstract
Following intravenous injection of 17 β-estradiol-6,7- 3H-17-glucosiduronate into a normal human female the urinary conjugated forms of labelled estrone and 17 β-estradiol were identified by DEAE-Sephadex and Celite partition chromatography; comparison with certain authentic 14C-labelled conjugates; enzyme hydrolysis; crystallization with carrier steroids, including derivative formation. The main compounds identified were estrone-3-glucosiduronate, 17 β-estradiol-17-glucosiduronate and 17 β-estradiol-3-glucosiduronate in the approximate proportions of 5.6:3.7:1. Some evidence was also obtained for the presence of very small amounts of estrone-3-sulfate and 17 β-estradiol-3-sulfate-17-glucosiduronate.
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