Abstract
The aim was to investigate the potential contribution of a major birch pollen Betv1-homologous allergen to birch pollen-associated tomato fruit allergy. Two isoforms of a Betv1-homologous protein (designated Solal4) from tomato fruit were identified by cDNA-cloning and produced as recombinant proteins. Allergen-specific IgE levels to tomato, birch pollen, Betv1, and Sola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Solal4 was recognized in 76% of birch/tomato allergic patients, while tomato- and Betv1-specific IgE was detectable in 64% and 81% of sera. Almost all patients sensitized to Betv1 reacted with Solal4. Both Solal4 isoforms displayed allergenic potency and IgE-cross-reactivity with Betv1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse. Sola l 4, a novel pathogenesis related-10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as classII allergen. IgE-testing using Sola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component-resolved allergy diagnosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
