Abstract
We have investigated the applicability of TLC/scanning densitometry (TLC/SCAN) to the analysis of small amounts of coal tar dyes in foods. The use of a combination of reversed-phase C18 TLC and sample concentration techniques on the TLC plate enabled us to measure the visible absorption spectra of small amounts of coal tar dyes in foods with good accuracy. The sample concentration techniques improved the detection limits 10-20-fold. The TLC/SCAN was successfully applied to the identification of coal tar dyes in 48 food samples which were suspected to be in violation of the Japanese Food Sanitation Law. The present method is convenient for the rapid identification of small amounts of coal tar dyes in foods.
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