Abstract
BackgroundBruchid beetles are an important storage pest of grain legumes. Callosobruchus sp. infect mungbean (Vigna radiata) at low levels in the field, multiply during grain storage and can destroy seed stocks in a few months. Resistance against bruchid beetles has been found in wild mungbean V. radiata var. sublobata TC1966 and in cultivated mungbean line V2802.ResultsBruchid resistance data were obtained from recombinant inbred line populations TC1966 (V. radiata var. sublobata) × NM92 (F12) and V2802 (V. radiata) × NM94 (F7). More than 6,000 single nucleotide polymorphic markers were generated through genotyping by sequencing (GBS) for each of these populations and were used to map bruchid resistance genes. One highly significant quantitative trait locus (QTL) associated with bruchid resistance was mapped to chromosome 5 on genetic maps of both populations, suggesting that TC1966 and V2802 contain the same resistance locus. Co-segregation of all markers associated with resistance indicated the presence of only one major resistance QTL on chromosome 5, while QTL analysis based on physical map positions of the markers suggested the presence of multiple QTLs on different chromosomes. The diagnostic capacity of the identified molecular markers located in the QTL to correctly predict resistance was up to 100 %.ConclusionsMolecular markers tightly linked to bruchid resistance loci of two different mungbean resistance sources were developed and validated. These markers are highly useful for developing resistant lines.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0847-8) contains supplementary material, which is available to authorized users.
Highlights
Bruchid beetles are an important storage pest of grain legumes
Sixty-one F12 families of TC1966 × NM92 were tested for bruchid resistance
The segregation pattern suggested the action of a major resistance gene supported by genes modulating resistance, explaining the presence of intermediate resistant phenotypes in homozygous recombinant inbred line (RIL) families
Summary
Bruchid beetles are an important storage pest of grain legumes. Callosobruchus sp. infect mungbean (Vigna radiata) at low levels in the field, multiply during grain storage and can destroy seed stocks in a few months.Resistance against bruchid beetles has been found in wild mungbean V. radiata var. sublobata TC1966 and in cultivated mungbean line V2802. Bruchid beetles are an important storage pest of grain legumes. Mungbean (Vigna radiata) is cultivated on about 6 million hectares, mainly in Asia. It is consumed as grains or as sprouts, the green pods are eaten as a vegetable, and it is processed into a variety of products such as noodles, sweets or drinks. Mungbean fixes and adds nitrogen to the soil, which benefits the subsequent crop. Despite these benefits, expansion of the mungbean growing area is limited, mainly due to diseases and pests affecting the crop and reducing yield and profitability
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