Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck

Abstract

Prolactin plays important roles in avian reproduction as it induces broody behavior and regulates follicular development. The objectives of this study were to detect Single Nucleotide Polymorphisms (SNPs) of prolactin gene in Alabio and Mojosari ducks and determine the restriction enzymes for genotyping. Genomic DNA was isolated from 50 blood samples per each Alabio and Mojosari ducks. PCR amplification and sequencing were carried out to identify the SNP. In this study, SNP T-6068C was found based on three GenBank sequences alignment. Two SNPs (C-5796A and T-5817C) in intron 4 region were detected based on sequences of Alabio and Mojosari PCR products. Enzymes Fok1, BtsCI, BsrI were detected to recognize the SNP C-5796A. SNP T-6068C can be digested by enzym TspDTI. However no enzym was detected to recognize SNP T-5817C. In conclusion the SNPs detected from this study may be used in future studies to investigate the association of prolactin gene and egg production traits in Alabio and Mojosari ducks.