Abstract
Several representative glycoproteins have been stained with the cationic carbocyanine dye, “Stains-all,” after electrophoretic separation in polyacrylamide gels. Sialoglycoproteins are indicated when a protein with a positive periodic acid-Schiff (PAS) reaction stains blue with “Stains-all” and stains red-purple after neuraminidase digestion. The enzymatic digestion is necessary to identify the acidic portion of the molecule as sialic acid since other acidic macromolecules such as nucleic acids, phosphopoproteins, and glycosaminoglycans stain shades of blue with this dye. The procedure has been compared with the PAS method. It offers advantages for the staining of glycoproteins rich in sialic acids in terms of sensitivity, simplicity, and time required.
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