Abstract
This research is an activity to characterize and determine several types of soil-borne fungi based on the use of DNA sequences. This study aims to determine the species name and genealogy of four selected fungal isolates from the genus Aspergillus isolated from horticultural fields in Bumiaji, Batu, East Java. The identification of biocontrol agents begins with macroscopic and microscopic morphological observations, followed by DNA isolation, polymerase chain reaction application using ITS 1 and ITS 4 primers. Sequencing is done using Sanger sequencing. The nucleotides generated from the sequencer machine (ABI 3730XL sequencer) were compared with the gene bank using the BLAST-NCBI program, and their relationships were analyzed using MEGA X software. The results showed that based on BLAST analysis of the nucleotide sequences of Aspergilus spp. isolates 6, 7, 17, and 21 were compatible with Aspergillus tamarii with 100% Query Cover and 100% identical rate. These four Aspergillus isolates have the potential as bofertilizer agents and can be used for various industrial purposes.
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