Identification of Self-Incompatibility Genotypes in Some Sand Pears ( Pyrus pyrifolia Nakai) by PCR-RFLP Analysis

Abstract

The identification of self-incompatibility genotype ( S-genotype) will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S- RNase sequencing. The S-genotypes of these cultivars were determined as follows: Huali 1 S 1 S 3 , Shounan S 1 S 3 , Xizilü S 1 S 4 , Qingxiang S 3 S 7 , Sanhua S 2 S 7 , Huangmi (Imamuranatsu) S 1 S 6 , Huali 2 S 3 S 4 , Baozhuli S 7 S 33 , Cangxixueli S 5 S 15 . S- RNase alleles ( S 1 to S 9 ) in sand pear could be identified effectively by PCR-RFLP analysis.