Identification of Secondary Metabolite Gene Clusters in the Genome of Bacillus pumilus Strains 7P and 3-19

Abstract

Members of Bacillus pumilus species are industrially important microorganisms able to secrete a wide range of hydrolytic enzymes and secondary metabolites. However, the traditional methods (based on phenotypes) used to identify such products are time-consuming and are not very specific. Currently, the availability of whole genome sequencing and bioinformatics tools has become a powerful approach for identification of gene clusters associated with the synthesis of substances useful for biotechnology. In order to estimate the potential industrial significance and application of B. pumilus, genome sequencing of two strains (7P and 3-19) and searching of secondary metabolite clusters were performed using antiSMASH program. These analyses revealed 11 potential gene clusters for the synthesis of bacilysin, lichenysin, bacteriocin, and other substances.