Identification of S-genotypes and novel S-RNase alleles in Prunus mume

Abstract

SummaryPrunus mume (Japanese apricot) cultivars are self-incompatible and orchards require pollinator cultivars to guarantee optimal fruit production. Identification of the self-incompatibility (S) genotypes of P. mume cultivars is therefore useful for choosing pollinators and for breeding. To elucidate the S-genotypes of eleven Chinese and one introduced cultivar, PCR was performed with primers amplifying the variable second intron of the S-RNase gene. The S-genotyping results were as follows: S4S12 for ‘Dantaofen’; S3S4 for ‘Duoezhusha’; S12S13 for ‘Fubantiaozhi’; S15S16 for ‘Hongding’; S13S14 for ‘Longyanmei’; S5S11 for ‘Musashino’; S10S14 for ‘Xiaoyezhugan’; and S3S15 for ‘Qingfeng’,‘Qingjia’, ‘Xiyeqing’, ‘Dayeqing’ and ‘Changnong 17’. We identified seven new S-RNase alleles, S10-RNase to S16-RNase, deposited in GenBank under the accession numbers: DQ011150, DQ201191, DQ201192, DQ345781, DQ768219, DQ903312 and EF990750, respectively.