Abstract
Restriction fragment length polymorphisms (RFLP) were investigated as a means of identifying rose ( Rosa × hybrida) cultivars for patent protection. A library of genomic DNA was generated by shotgun cloning HindIII digested DNA fragments of the rose cultivar ‘Confection’ into the plasmid pUC8. Clones with low copy number sequences were identified and used as probes in Southern hybridization analyses of restriction digested DNA from different rose cultivars. We have identified 15 probes that display RFLP useful in cultivar identification. One of these probes, pH1.3C, can identify 12 different cultivars. Based on hybridizations with probes pH1.3C and pH1.6C, a total of 16 cultivars have been distinguished from one another. Using different restriction enzyme and probe combinations, we expect to distinguish many more rose cultivars.
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