Identification of Romanian Vitis vinifera L. cultivars in must using nuclear microsatellite markers.

Abstract

Accurate identification of grapevine cultivars is important for Romanian wine- makers and the extrapolation of the grapevine ident ification methods into musts is particularly releva nt in controlling the quality and authenticity of arom atic and high quality red wines. In this study, molecular SSR (Simple Sequence Repeats) analysis of Vitis vinifera L. cultivars (Busuioac a de Bohotin, Tamâioas a Româneasc a, Negru Aromat, Feteasc a Neagr a, Negru de Dr agas ani, Amurg and Novac) was applied in order to detect and correctly identify the grapevine cultivars present in monovarietal musts. DNA provided from each analyzed cultivar was successful extracted from leaves and must at the beginning of the fermen tation (day 1) using CTAB method and amplicons generated from eight SSR primers (VVS2, VVS5, VVS29, MD5, MD7, ZAG 47, ZAG 62, ZAG 79) were analyzed. Results shows that, at molecular lev el, there were no differences between the corresponding leaf and varietal must profiles and S SR markers can be successful used for characterisation and differentiation of analyzed gr apevine cultivars identified in musts.