Identification of rice sheath blight and blast quantitative trait loci in two different O. sativa/O. nivara advanced backcross populations

Abstract

Two accessions of Oryza nivara, a wild ancestral species of rice (O. sativa) identified as being moderately resistant to sheath blight and leaf blast disease, were used as donor parents to develop two advanced backcross populations with the US rice cultivar, Bengal, as the recurrent parent. The O. nivara donor parent for Wild-1 (252 BC2F1 lines) was acc. IRGC100898 and for Wild-2 (253 BC2F1 lines) was acc. IRGC104705. Both populations were genotyped with 131 simple sequence repeat markers and the linkage maps covered 1,567.5 cM (Wild-1) and 1,312.2 cM (Wild-2). Sheath blight (ShB) disease was evaluated in both inoculated greenhouse and field conditions. Days to heading (DH), plant height (PH), and plant type (PT), confounding factors for sheath blight disease under field conditions, were recorded. Leaf blast disease was rated under inoculated greenhouse conditions. Multiple interval mapping identified qShB6 with resistance to sheath blight disease attributed to the O. nivara parent in the greenhouse. In the field, qShB6 also was the most significant ShB quantitative trait locus (QTL) in all trials, with resistance attributed to O. nivara. In addition, qShB1 and qShB3 were identified in all trials but were not always attributed to the same parent. The qShB6 QTL is in the same region as the DH-QTL, qDH6, and qShB1 is in the same region as the major PH-QTL, qPH1, suggesting that these ShB-QTL may be confounded by other traits. Although qShB3 did not have as large an effect as other loci, it was not confounded by either DH or PH. For leaf blast, qBLAST8-1 was found in both populations providing resistance to races IB1 and IB49, whereas qBLAST12 providing resistance to both races, was only found in Wild-2. Resistance was attributed to O. nivara for both QTL, and blast resistance genes have been previously reported in these regions.