Abstract
Periplasmic oligopeptide binding protein (OppA) is part of a multimeric cytoplasmic membrane protein complex, whose function is known as peptide transporters found in Gram-negative bacteria. A chaperone-like activity has been found for the OppA from Yersinia pseudotuberculosis, using biochemical experiments. Through computational analysis, we selected two amino acid residues (R41 and D42) that probably are involved in the chaperone-like activity. Our results to corroborate how OppA assists refolding and renaturation of lactate dehydrogenase and alpha-glucosidase denatured enzymes.
Highlights
The pentameric OppA/B/C/D/F is part of a huge protein ATP-binding cassette (ABC) system of related transporters to take up peptides into the cells
Prior to selecting the residues to be replaced, a possible mechanistic role for refolding mechanism was discussed for heat shock proteins (HSP) in general, and especially the case of small HSPs (Skorko-Glonek et al 2007; Matsuzaki et al 2000), PDB code: 5D4W (Heuck et al 2016) and PDB code: 3AAC (Takeda et al 2011)
We tested the ability of the OppA protein to refold proteins denatured with chaotropic agents such as guanidine-hydrochloride and urea
Summary
The pentameric OppA/B/C/D/F is part of a huge protein ATP-binding cassette (ABC) system of related transporters to take up peptides into the cells. The complex embraces at the extracellular side our target protein OppA, which itself is associated with two pore-forming transmembrane proteins (OppB and OppC) as well as two nucleotide-binding proteins (OppD and OppF) which function as ATPases (Monnet 2003). OppA binds to an incoming oligopeptide and delivers it to the import complex in the inner membrane, which requires ATP hydrolysis to transport the oligopeptide into the cell. The OppA protein possess versatility in substrate recognition, Upon genetical manipulations Lennon and coworkers found the periplasm enriched with our target OppA among others like DppA, Ivy, OsmY or HdeB. The researchers could test the chaperone-like activity in vitro with positive results for OppA and closely related DppA (Lennon et al 2015)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
