Abstract
Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertility-restoring gene(Rf i) of upland cotton. A total of 425 arbitrary 10-mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC3 segregating population of (0-613-2R X Simian No. 3). Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of (Zhong 12A-1 × 0-613-2R)F2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV-15300 was linked with the fertility-restoring gene (Rf1) with a recombination value of 13.0±2.57%.
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