Identification of QTNs, QTN-by-environment interactions and genes for yield-related traits in rice using 3VmrMLM.

Abstract

Rice, which supports more than half the population worldwide, is one of the most important food crops. Thus, potential yield-related quantitative trait nucleotides (QTNs) and QTN-by-environment interactions (QEIs) have been used to develop efficient rice breeding strategies. In this study, a compressed variance component mixed model, 3VmrMLM, in genome-wide association studies was used to detect QTNs for eight yield-related traits of 413 rice accessions with 44,000 single nucleotide polymorphisms. These traits include florets per panicle, panicle fertility, panicle length, panicle number per plant, plant height, primary panicle branch number, seed number per panicle, and flowering time. Meanwhile, QTNs and QEIs were identified for flowering times in three different environments and five subpopulations. In the detections, a total of 7~23 QTNs were detected for each trait, including the three single-environment flowering time traits. In the detection of QEIs for flowering time in the three environments, 21 QTNs and 13 QEIs were identified. In the five subpopulation analyses, 3~9 QTNs and 2~4 QEIs were detected for each subpopulation. Based on previous studies, we identified 87 known genes around the significant/suggested QTNs and QEIs, such as LOC_Os06g06750 (OsMADS5) and LOC_Os07g47330 (FZP). Further differential expression analysis and functional enrichment analysis identified 30 candidate genes. Of these candidate genes, 27 genes had high expression in specific tissues, and 19 of these 27 genes were homologous to known genes in Arabidopsis. Haplotype difference analysis revealed that LOC_Os04g53210 and LOC_Os07g42440 are possibly associated with yield, and LOC_Os04g53210 may be useful around a QEI for flowering time. These results provide insights for future breeding for high quality and yield in rice.