Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating fungal diseases of wheat worldwide. It is essential to discover more sources of stripe rust resistance genes for wheat breeding programs. Specific locus amplified fragment sequencing (SLAF-seq) is a powerful tool for the construction of high-density genetic maps. In this study, a set of 200 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Chuanmai 42 (CH42) and Chuanmai 55 (CH55) was used to construct a high-density genetic map and to identify quantitative trait loci (QTLs) for stripe rust resistance using SLAF-seq technology. A genetic map of 2828.51 cM, including 21 linkage groups, contained 6732 single nucleotide polymorphism markers (SNP). Resistance QTLs were identified on chromosomes 1B, 2A, and 7B; Qyr.saas-7B was derived from CH42, whereas Qyr.saas-1B and Qyr.saas-2A were from CH55. The physical location of Qyr.saas-1B, which explained 6.24–34.22% of the phenotypic variation, overlapped with the resistance gene Yr29. Qyr.saas-7B accounted for up to 20.64% of the phenotypic variation. Qyr.saas-2A, a minor QTL, was found to be a likely new stripe rust resistance locus. A significant additive effect was observed when all three QTLs were combined. The combined resistance genes could be of value in breeding wheat for stripe rust resistance.
Highlights
Bread wheat (Triticum aestivum) is one of the most important food crops for mankind and the security of wheat production benefits economic development and social stability
Compared with the standard Fluorescence In Situ Hybridization (FISH) karyotype of wheat and rye chromosomes indicated by Tang et al [19], we found that Chuanmai 55 (CH55) carried a pair of 1RS·1BL translocation and two pairs of 5B–7B reciprocal translocation chromosomes, whereas Chuanmai 42 (CH42) had a normal wheat karyotype (Figure 1)
Qyr.saas-7B was derived from CH42, whereas the other two quantitative trait loci (QTL) were from CH55
Summary
Bread wheat (Triticum aestivum) is one of the most important food crops for mankind and the security of wheat production benefits economic development and social stability. Among the formally designated stripe rust resistance genes, 55 confer seeding resistance and 24 genes are described as APR genes [6,7,8,9,10] Some of these genes have been very widely deployed in agriculture by major epidemics following the emergence and increase of new virulent pathogen races. Examples of such occurrences of “boom and bust” situations in China include the use of Yr1 from the 1950s, Yr9 from the 1970s (overcome by Chinese yellow rust race CYR29), so-called Fan 6 resistance from the 1990s (race CYR31), and Yr24/Yr26 from the 2000s (CYR34). It is necessary to find new sources of stripe rust resistance to identify the underlying genes for resistance and to convince breeders that they are worthy of use in current breeding programs
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