Abstract
Anthracnose disease caused by a fungus Colletotrichum gloeosporioides is the primary cause of yield loss in water yam (Dioscorea alata), the widely cultivated species of yam. Resistance to yam anthracnose disease (YAD) is a prime target in breeding initiatives to develop durable-resistant cultivars for sustainable management of the disease in water yam cultivation. This study aimed at tagging quantitative trait loci (QTL) for anthracnose disease resistance in a bi-parental mapping population of D. alata. Parent genotypes and their recombinant progenies were genotyped using the Genotyping by Sequencing (GBS) platform and phenotyped in two crop cycles for two years. A high-density genetic linkage map was built with 3184 polymorphic Single Nucleotide Polymorphism (NSP) markers well distributed across the genome, covering 1460.94 cM total length. On average, 163 SNP markers were mapped per chromosome with 0.58 genetic distances between SNPs. Four QTL regions related to yam anthracnose disease resistance were identified on three chromosomes. The proportion of phenotypic variance explained by these QTLs ranged from 29.54 to 39.40%. The QTL regions identified showed genes that code for known plant defense responses such as GDSL-like Lipase/Acylhydrolase, Protein kinase domain, and F-box protein. The results from the present study provide valuable insight into the genetic architecture of anthracnose resistance in water yam. The candidate markers identified herewith form a relevant resource to apply marker-assisted selection as an alternative to a conventional labor-intensive screening for anthracnose resistance in water yam.
Highlights
Yam (Dioscorea spp.) is a multi-species monocotyledonous crop widely grown in the tropics and subtropics [1]
This study selected two parents based on their responses to yam anthracnose disease and created their F1-derived recombinant clonal population to assess the functional association of anthracnose resistance and genetic markers using the quantitative trait loci (QTL) mapping approach
This study developed a highly saturated and accurate genetic linkage map for water yam
Summary
Yam (Dioscorea spp.) is a multi-species monocotyledonous crop widely grown in the tropics and subtropics [1]. It is the most valuable crop in West Africa, where its cultivation began 11,000 years ago [2]. Of the over 600 yam species, water yam (D. alata) is an extensively cultivated species worldwide [3]. In Africa, white Guinea yam (D. rotundata) is the most cultivated yam species, followed by water yam [3]. Yam production in West Africa is mainly by smallholder farmers, making it a significant source of farm employment and income for this group. Yam plays a vital role in traditional medicine and the socio-cultural life of the people as it is involved in many key life ceremonies [4]
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