Abstract
Increasing seed oil content is one of the most important goals in breeding of rapeseed (B. napus L.). To dissect the genetic basis of oil content in B. napus, a large and new double haploid (DH) population containing 348 lines was obtained from a cross between ‘KenC-8’ and ‘N53-2’, two varieties with >10% difference in seed oil content, and this population was named the KN DH population. A genetic linkage map consisting of 403 markers was constructed, which covered a total length of 1783.9 cM with an average marker interval of 4.4 cM. The KN DH population was phenotyped in eight natural environments and subjected to quantitative trait loci (QTL) analysis for oil content. A total of 63 identified QTLs explaining 2.64–17.88% of the phenotypic variation were identified, and these QTLs were further integrated into 24 consensus QTLs located on 11 chromosomes using meta-analysis. A high-density consensus map with 1335 marker loci was constructed by combining the KN DH map with seven other published maps based on the common markers. Of the 24 consensus QTLs in the KN DH population, 14 were new QTLs including five new QTLs in A genome and nine in C genome. The analysis revealed that a larger population with significant differences in oil content gave a higher power detecting new QTLs for oil content, and the construction of the consensus map provided a new clue for comparing the QTLs detected in different populations. These findings enriched our knowledge of QTLs for oil content and should be a potential in marker-assisted breeding of B. napus.
Highlights
Rapeseed (B. napus, AACC, 2n = 38) is the world’s second most important oilseed crop after soybean
Because of a strong requirement for vernalization, the highoil parent ‘N53-2’ and some double haploid (DH) lines did not flower in the spring rapeseed area (10GS and 11GS) and these lines were treated as missing data
Seed oil content is an important trait for B. napus, controlled by complex genetic architecture and influenced by environment
Summary
Rapeseed (B. napus, AACC, 2n = 38) is the world’s second most important oilseed crop after soybean. With the increasing demand for vegetable oil and canola-oil-based biodiesel usage, oil content has become a key factor for increasing oil production. Better understanding of the genetic determinants of this trait is very important in breeding B. napus for oil content. QTL mapping is an effective approach to dissect the genetic mechanisms of complex quantitative traits [5], and many QTLs for oil content or fatty acid composition have been detected in various crops, such as maize [6,7,8], soybean [9,10,11], sunflower [12,13], peanut [14,15] and B. juncea [16,17]
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