Identification of QTL for Fiber Quality and Yield Traits Using Two Immortalized Backcross Populations in Upland Cotton.

Hantao Wang,Beibei Zhang,Dingguo Li,Zhongxu Lin,Baosheng Dai,Chao Shen,Cong Huang,Wenxia Zhao,David D Fang

doi:10.1371/journal.pone.0166970

Hantao Wang, Beibei Zhang + Show 7 more

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https://doi.org/10.1371/journal.pone.0166970

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Abstract

Two immortalized backcross populations (DHBCF1s and JMBCF1s) were developed using a recombinant inbred line (RIL) population crossed with the two parents DH962 and Jimian5 (as the males), respectively. The fiber quality and yield component traits of the two backcross populations were phenotyped at four environments (two locations, two years). One hundred seventy-eight quantitative trait loci (QTL) were detected including 76 for fiber qualities and 102 for yield components, explaining 4.08–17.79% of the phenotypic variation (PV). Among the 178 QTL, 22 stable QTL were detected in more than one environment or population. A stable QTL, qFL-c10-1, was detected in the previous F2 population, a RIL population in 3 environments and the current two BCF1 populations in this study, explaining 5.79–37.09% of the PV. Additionally, 117 and 110 main-effect QTL (M-QTL) and 47 and 191 digenic epistatic QTL (E-QTL) were detected in the DHBCF1s and JMBCF1s populations, respectively. The effect of digenic epistasis played a more important role on lint percentage, fiber length and fiber strength. These results obtained in the present study provided more resources to obtain stable QTL, confirming the authenticity and reliability of the QTL for molecular marker-assisted selection breeding and QTL cloning.

Highlights

Cotton is the largest natural textile fiber provider and one of the important oil crops worldwide
In the JMBCF1s, the minimum phenotype data except FL in 2013HG was smaller than those in the parent Jimian5. These results showed that all traits performed transgressive segregation in the two BCF1 populations
A recombinant inbred line (RIL) population was crossed with the two parents (DH962 and Jimian5) as the males to construct two immortalized BCF1 populations

Summary

Introduction

Cotton is the largest natural textile fiber provider and one of the important oil crops worldwide. 50 species have been discovered in the cotton genus (Gossypium), among which only four cultivated species (G. herbaceum, G. arboreum, G. hirsutum, and G. barbadense) could be used for fiber production [1]. Of the four cultivated species, G. hirsutum, known as upland cotton, is widely cultivated because of its wide adaptability and high production, accounting for over 95% of the world’s cotton production [2]. Cotton fiber is an important raw material for the textile industry because of its softness and comfort; the products of cotton fiber are very popular [3]. With the stricter requirements of PLOS ONE | DOI:10.1371/journal.pone.0166970. With the stricter requirements of PLOS ONE | DOI:10.1371/journal.pone.0166970 December 1, 2016

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