Abstract
Switchgrass (Panicum virgatum L.) is a warm-season perennial grass species that is utilized as forage for livestock and biofuel feedstock. The stability of biomass yield and regrowth vigor under changing harvest frequency would help manage potential fluctuations in the feedstock market and would provide a continuous supply of quality forage for livestock. This study was conducted to (i) assess the genetic variation and (ii) identify the quantitative trait loci (QTL) associated with regrowth vigor after multiple cuttings in lowland switchgrass. A nested association mapping (NAM) population comprising 2000 pseudo F2 progenies was genotyped with single nucleotide polymorphism (SNP) markers derived from exome-capture sequencing and was evaluated for regrowth vigor in 2017 and 2018. The results showed significant variation among the NAM families in terms of regrowth vigor (p < 0.05). A total of 10 QTL were detected on 6 chromosomes: 1B, 5A, 5B, 6B, 7B, and 8A, explaining the phenotypic variation by up to 4.7%. The additive genetic effects of an individual QTL ranged from −0.13 to 0.26. No single QTL showed a markedly large effect, suggesting complex genetics underlying regrowth vigor in switchgrass. The homologs of candidate genes that play a variety of roles in developmental processes, including plant hormonal signal transduction, nucleotide biosynthesis, secondary metabolism, senescence, and responses to both biotic and abiotic stresses, were identified in the vicinity of QTL.
Highlights
Switchgrass (Panicum virgatum L.) is a warm-season, perennial grass that is recognized as an important component of North American tallgrass prairies [1]
A notable variation in the regrowth vigor among the nested association mapping (NAM) families was observed, demonstrating the opportunity to improve this trait by exploiting additive genes
Our study suggests that the regrowth vigor of switchgrass is a complex trait that involves both additive and dominant gene action
Summary
Switchgrass (Panicum virgatum L.) is a warm-season, perennial grass that is recognized as an important component of North American tallgrass prairies [1]. The natural population of switchgrass is reported to exist across a broad range of environments. Based on the natural habitat, switchgrass cultivars are mainly classified into two distinct ecotypes: lowland and upland [2,3]. Lowland ecotypes are characterized as having taller plants, thicker stems, fewer tillers, and are adapted to relatively warm and wet environments compared to upland ecotypes [1]. Lowland ecotypes are mostly tetraploids (2n = 4x = 36), whereas upland ecotypes are predominantly octaploids (2n = 8x = 72) [4]
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