Abstract
This chapter explains the identification of putative precursors of ovalbumin and ovomucoid messenger rRNAs. In an experiment described in the chapter, specific DNA probes prepared from structural and intervening sequences within the natural ovalbumin gene were used to identify the precursors of ovalbumin mRNA. Nuclear RNA from hormone-stimulated chick oviducts was electrophoresed under denaturing condition followed by transfer to diazobenzyloxymethyl paper, and hybridization to 32 p-DNA probes for structural sequence probes to cytoplasmic RNA gave a single low molecular weight band at the expected size of mature ovalbumin mRNA. Hybridization of the same probe to nuclear RNA demonstrated multiple species of RNA that are higher in molecular weight than mature ovalbumin rRNA. The largest molecule has a molecular weight of more than 8000 nucleotides in length, which could accommodate the entire natural ovalbumin gene sequence. These high-molecular-weight species contain RNA complementary to both structural and intervening sequences of the ovalbumin gene and, thus, appear to represent ovalbumin gene transcripts at various processing stages.
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