Abstract
The yellow peach moth, Conogethes punctiferalis, is an extremely important polyphagous insect in Asia. The chemosensory systems of moth play an important role in detecting food, oviposition sites and mate attraction. Several antennal chemosensory receptors are involved in odor detection. Our study aims to identify chemosensory receptor genes for potential applications in behavioral responses of yellow peach moth. By transcriptomic analysis of male and female antennae, 83 candidate chemosensory receptors, including 62 odorant receptors, 11 ionotropic receptors and 10 gustatory receptors were identified. Through Blast and sequence alignment, the highly conserved co-receptor Orco was annotated, eight unigenes clustered into pheromone receptors, and two clustered as sugar receptor. Among the IRs, one unigenes was similar with co-receptors IR25a. Expression levels of 50 odorant receptors were further evaluated by quantitative real-time PCR in antennae. All the ORs tested were detected in antennae and some of which were associated with sex-biased expression. The chemosensory receptors identified in C. punctiferalis provide a foundational resource for further analysis on olfaction for behavior. The expression profiles of ORs in antennae indicated variant functions in olfactory recognition, and our results provided the possibility for the potential application of semiochemical to control this pest moth.
Highlights
BmOR47 respond to benzoic acid, 2-phenylethanol, and benzaldehyde respectively[29]
Few olfactory receptor genes have been reported in this moth[44], more receptor genes investigated allows a better understanding of the molecular basis of olfaction
From the transcriptome of C. punctiferalis, we identified 83 chemoreceptors including 62 ORs, 11 IRs and 10 GRs
Summary
BmOR47 respond to benzoic acid, 2-phenylethanol, and benzaldehyde respectively[29]. While PRs (BmOR1 and BmOR3) respond to bombykol (E10Z12-16: OH) and bombykal (E10Z12-16: Ald) respectively, which are major and minor sex pheromone components in B. mori.[30,31]. The GRs are generally expressed in gustatory receptor neurons (GRNs) within gustatory organs. Some GRs expressed in antennae cannot identify volatile odorants, but respond to carbon dioxide[37]. Based on the expression location, two subfamily of IRs are identified: conserved antennal IRs and divergent IRs, indicating the function of IRs in olfactory and taste[39]. Few olfactory receptor genes have been reported in this moth[44], more receptor genes investigated allows a better understanding of the molecular basis of olfaction. In total 83 chemoreceptor genes including 62 ORs, 11 IRs and 10 GRs were identified. The results assist with the identification of genes involved in C. punctiferalis olfactory, help better understand the gene expression in sex, and provide the molecular basis for further study of pheromone and host volatile recognition
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