Abstract
The importance of examining environmental stresses and their role in predicting and evaluating the growth and yield of crops is very evident. Environmental stresses are the most important factor in reducing agricultural yields worldwide. In order to evaluate the environmental impact of water loss on the amount of proteins affected in sunflower, an experiment was conducted in randomized complete block design with three replications at Karaj Oil Seeds Research Institute. In order to study the response of susceptible line (BGK221) and tolerant line (RGK46) under drought stress conditions, extraction of protein by acetone deposition method was performed in 8 leaves and of 3 seedlings in each replication. The amount of extracted protein was determined by Bradford method using two-dimensional electrophoresis and existence of significant difference between the bands in sensitive and tolerant lines was investigated. A total of 467 repeatable bands were found in the tolerant line and 417 repeatable bands appeared in the sensitive line. Among these proteins, 6 bands in tolerant line (No 503, 1901, 904, 3301, 7011, 9005) and 6 bands in sensitive line (No 704, 811, 3205, 4108, 7307, and 9207) were significantly affected by drought stress. In both sensitive and tolerate lines the main consequence is increase in amount of protein. The results showed that the most important factor of tolerant line adaptive for environmental stress conditions is maintaining normal cell metabolism, keeping moisture in the cell, strengthening cellular structure and antioxidant defense. The study also showed that drought stress had the greatest effect on cytoplasmic and nucleus proteins, metabolism and energy of proteins
Highlights
2 MATERIALS AND METHODSPlants need the presence of nutrients in the soil, protection against pests and diseases, and the presence of appropriate environmental factors to grow well and maintain their health (Maghsoudi, 2006)
When plants are exposed to environmental stress, they respond to stress at the plant, cell, or molecule level
Plants to counteract or reduce the effects of drought stress may change the pattern of gene response or the amount of proteins within the tissue (Kanlaya et al, 2005)
Summary
Plants need the presence of nutrients in the soil, protection against pests and diseases, and the presence of appropriate environmental factors to grow well and maintain their health (Maghsoudi, 2006). Proteomics is referred as the analysis of a collection of proteins encoded by the genome (Karam et al, 2007) This technology provides quantitative and qualitative analysis of a large number of proteins; unlike the genome, which is a constant and unchanging nature, the proteome is fundamentally dynamic and variable, the proteome differs from organism to organism according to the type of tissue, cell, organ and environmental conditions (Goksoy et al, 2004). Sensitive line (BGK221) and drought tolerant line (RGK46) were identified For this purpose, sunflower inbred lines were cultivated in two separate experiments under normal irrigation and drought stress conditions in a randomized complete block design with three replications. Significant differences between bandsof sensitive and tolerant lines were investigated by t-test
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
