Identification of protein profiles of Escherichia coli, Staphylococcus aureus and their corresponding phages

Abstract

The present study was carried out for the assessment and detecting of the Escherichia coli and Staphylococcus aureus bacteriophages and their bacterial host’s proteins includes visual inspection and comparison of their molecular weight mass. Systematic methods that use microorganisms (viruses and bacteria) to test samples from the local sewage water have been developed but require some change in the classical culturing of the organism to detect it easily by naked eye through the changes in selective media. We have developed a Sodium Dodecyl Sulphate Ploy Acrylamide Gel Electrophoresis (SDS PAGE) assay for bacteriophage samples preparation that allows the rapid detection of proteins bands of these viruses by using freeze drying technique. We found that the protein profiles of E. coli bacteriophage showed three major bands with molecular weight mass of 47, 35 and 16 kilo Dalton and 34 and 20 kDa for S. aureus phage, the band of 35 kDa was the common shared peak between the phage and the bacterial host due to the bacteriophage lytic cycle. The method is suitable for use with both the bacteriophages and their bacterial hosts. The assay is rapid, sensitive, and inexpensive; requires only small sample volumes; and can be automated.