Identification of Protein Partners by APEX2 Proximity Labeling.

Abstract

Proximity labeling methods enable the identification of proteins in the vicinity of a protein of interest in living cells. Among them, APEX2 proximity is a powerful method to spatiotemporally define in vivo "proxisomes" in dynamic bacterial protein systems. Here we describe a standardized APEX2 proximity labeling protocol and possible adaptations to capture protein partners in native conditions.