Identification of prostaglandin receptors in human ureters

Matthias Oll,Turang E Behbahani,Stefan C Müller,Alexander Von Ruecker,Claudia Baumann,Jörg Ellinger

doi:10.1186/1471-2490-12-35

Matthias Oll, Turang E Behbahani + Show 4 more

Open Access

https://doi.org/10.1186/1471-2490-12-35

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Abstract

BackgroundProstaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter.MethodsThe expression pattern of PTGER1, PTGER2, PTGER3, PTGER4 and PTGFR was determined in human distal, mid and proximal ureter and renal pelvis samples using immunohistochemistry (protein levels) and quantitative real-time PCR (mRNA).ResultsPTGER1 was highly expressed in most samples irrespective of the ureteral localization; however, urothelial cells had higher levels of PTGER1 than smooth muscle cells. PTGFR was also moderately to strongly expressed in urothelial and smooth muscle cells. In comparison, PTGER2-4 expression was mostly unexpressed or weakly expressed in urothelial and smooth cells in all regions.ConclusionsOur data indicate high levels of PTGER1 in ureters.

Highlights

Prostaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter
The synthesis of prostaglandins in patients with obstructed ureters contributes to alterations in renal hemodynamic function during ureteral obstruction, recent studies indicate nonsteroidal anti-inflammatory drugs (NSAIDs) to be beneficial in these patients
COX2 mRNA has been shown to be upregulated in obstructed ureters and represents a valuable pharmaceutical target in patients with urolithiasis [2]

Summary

Introduction

Prostaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter. COX2 mRNA has been shown to be upregulated in obstructed ureters and represents a valuable pharmaceutical target in patients with urolithiasis [2]. Beside COX-inhibitors, prostanoids (PG) represent an estimable physiological and PTGFR (prostaglandin F2 alpha receptor) in urothelial and smooth muscle cells in the renal pelvis, proximal, mid and distal human ureter. High expression profiles of PTGER1 and PTGER3 in obstructed ureters could contribute to new treatment approaches, complementing the therapy of urolithiasis as an alternative to the widespread use of COX inhibitors to provide superior efficacy while minimizing potential side effects of current therapy strategies

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