Identification of presence of Candida albicans in primary root canal infections: An in vitro study

Abstract

Introduction: Microorganisms are recognized as the etiological agents for the majority of pulpal and periradicular diseases. Although bacteria are most researched, the contribution of fungi in endodontic infections is neglected. However, Candida albicans is the most commonly isolated fungi from the endodontic infections. Their identification is crucial for effective diagnosis and treatment planning. Hence, this study aimed to evaluate the presence of C. albicans in primary root canal infections using culture technique. Materials and Methods: Fifty root canal samples from primary endodontic infections were collected using file and paper point following disinfection protocol. Samples were inoculated in Sabouraud dextrose agar and incubated for 2–3 days. Taxonomy was evaluated using Gram-staining and germ tube test by macroscopic examination and optical microscopy. Results: Four out of 50 samples showed positive for C. albicans. Conclusion: This study confirmed the prevalence of 8% of C. albicans in primary endodontic infection.