Abstract
Colorectal carcinoma (CRC) is one of the most prevalent malignant tumors worldwide. Meanwhile, the majority of CRC related deaths results from liver metastasis. Gene expression profile of CRC patients with liver Metastasis was identified using 4 datasets. The data was analyzed using GEO2R tool. GO and KEGG pathway analysis were performed. PPI network of the DEGs between 1 and 2 gene sets was also constructed. The set 1 is named between primary CRC tissues and metastatic CRC tissues. The set 2 is named between primary CRC tissues and normal tissues. Finally, the prognostic value of hub genes was also analyzed. 35 DEGs (set 1) and 142 DEGs (set 2) were identified between CRC liver metastatic cancer patients. The PPI network was constructed using the top 10 set 1 hub genes which included AHSG, SERPINC1, FGA, F2, CP, ITIH2, APOA2, HPX, PLG, HRG and set 2 hub genes which included TIMP1, CXCL1, COL1A2, MMP1, AURKA, UBE2C, CXCL12, TOP2A, ALDH1A1 and PRKACB. Therefore, ITIH2 might represent the potential core gene for colon cancer liver metastasis. COL1A2 behaves as a key gene in colorectal carcinoma.
Highlights
Colorectal carcinoma (CRC) is one of the most prevalent malignant tumors worldwide
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to identify biological processes and pathways regulated by the differentially expressed genes (DEGs)
Data for expression profiles of 45,195 genes in CRC patients with liver metastasis in four datasets was downloaded from Gene Expression Omnibus (GEO) database
Summary
Colorectal carcinoma (CRC) is one of the most prevalent malignant tumors worldwide. the majority of CRC related deaths results from liver metastasis. PPI network of the DEGs between 1 and 2 gene sets was constructed. Abbreviations BP Biological process CC Cellular component CRC Colorectal Carcinoma DAVID Database for Annotation Visualization and Integrated Discovery DEG Differentially expressed gene GO Gene Ontology GEO Gene Expression Omnibus KEGG Kyoto Encyclopedia of Genes and Genomes MF Molecular function PPI Protein–protein interaction. We first identified differentially expressed genes (DEGs) between primary CRC and metastatic CRC tissues using data in the Gene Expression Omnibus (GEO) database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to identify biological processes and pathways regulated by the DEGs. PPI network of the top 10 most regulated hub genes related to liver metastasis in CRC. The prognostic utility of the hub genes in liver metastatic CRC patients was performed using GEPIA platform
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