Abstract
Abstract : The successful treatment of breast cancer requires detection of the disease at early stages. In this application, we propose to systematically isolate secreted and cell surface proteins (trafficked proteins) with increased expression in early stage breast tumors. These proteins are candidates for blood-borne markers and cell surface markers that can be used in the routine screening of early stage breast cancer. We proposed to first isolate all secreted and cell surface proteins from breast tumors of multiple patients, using a functional approach we have designed and validated. Next, the expression levels of these proteins in normal and early stage breast tumor tissues will be compared, and those with increased expression in tumors will be identified and analyzed. To date, a cDNA library has been constructed using human breast tumor samples. This library has been subjected to genetic screens to enrich cDNA fragments that encode signal peptides for trafficked protein. The library enriched for trafficked protein has been validated in preliminary analysis. DNA sequencing analysis is underway to further confirm the quality of the enriched library. Micro-array analysis will be carried out to identify the trafficked proteins with increased expression in human breast tumor tissues.
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