Identification of polymorphism in APAF1 gene in holstein cattle

Abstract

The article presents the data of DNA testing of Holstein cattle by the APAF1 gene. The structure of the Tatarstan population was studied in comparison with world experience. The aim of the study was to study the allelic polymorphism of the apoptosis peptide activating factor 1 gene (APAF1; g.C6315040T; p.Gln579Q→X), the cause of the fertility haplotype (HH1), in the domestic population of Holstein cattle of the Republic of Tatarstan. Genotyping was carried out by PCR-RFLP followed by electrophoretic separation in agarose gel in the presence of ethidium bromide. For the first time in the Republic of Tatarstan in the conditions of the Dairy farm "named Lenin” carried out the identification of cattle by the locus of APAF1 - BstC8 I, assessed the genetic equilibrium and structure of the population. As a result of gene diagnostics, two alleles and three genotypes were identified. The frequency of occurrence of alleles Q and X was 0.988 and 0.012; genotypes QQ and QX – 97.5 and 2.5%, respectively. Since homozygous XX embryos do not survive, they are never found among born animals. Chi-quad testing showed that the studied population is in genetic equilibrium according to the Hardy-Weinberg law. Our studies confirm an insignificant share of the presence of animals-carriers of the lethal allele in the population of Holstein cattle of domestic selection of the Republic of Tatarstan. In order to contain the spread of the mutant allele X of the apoptosis peptide activating factor 1 gene, it is recommended to conduct DNA testing of polymorphism of the APAF1 gene during the selection of pairs for breeding.