Abstract
As a folk medicinal plant, Juglans mandshurica has been used for the treatment of cancer in China and Korea. Traditionally, J. mandshurica is decocted together with chicken eggs. Both the decoction and medicated eggs possess anti-tumor properties. Clarifying the constituents of the decoction and absorbed by the medicated eggs is essential for the investigation of the active principles of J. mandshurica. Herein, the medicated eggs were prepared by decocting raw chicken eggs, having unbroken shells, with the decoction of J. mandshurica. A systematic investigation of the chemical profile of the J. mandshurica decoction and the medicated egg extraction was conducted by HPLC-Q-TOF-MS2. In total, 93 peaks, including 45 tannins, 14 naphthalene derivatives, 17 organic acids, 3 diarylheptanoids, 4 lignans, 3 anthraquinones, 1 flavonoid glycoside, 3 amino acids, and 3 nitrogenous compounds, were tentatively identified in the decoction. In the medicated egg extraction, 44 peaks including 11 organic acids, 3 amino acids, 3 nitrogenous compounds, 8 naphthalene derivatives, 3 diarylheptanoids, 15 tannins, and 1 lignan were tentatively identified. The chemical profile presented provided a detailed overview of the polar chemical constituents in J. mandshurica and useful information for the research of bioactive compounds of this plant.
Highlights
The Manchurian walnut, Juglans mandshurica Maxim., belongs to the Juglandaceae family and is mainly distributed in northern and northeastern China [1]
The absence of juglone in the decoction, the anti-tumor activity of the medicated eggs and the ambiguity of the active principles stimulated us to identify the chemical constituents in the decoction of J. mandshurica and those absorbed by the medicated eggs decocted with this plant
Data were acquired over a range of m/z 100–2000 for MS; 2 Data were acquired over a range of m/z 50–2000 for MS/MS; 3 Those compounds with stereo centers which configuration can’t be determined only by MS data was tentatively identified as the one that universally exist as natural products; 4 The absorption ration was calculated using the following formula: Peak area in medicated egg solution×0.1
Summary
The Manchurian walnut, Juglans mandshurica Maxim., belongs to the Juglandaceae family and is mainly distributed in northern and northeastern China [1]. The anti-tumor activity of this plant has been experimentally proven by pharmacological research As reported, both ethanol and water extracts of J. mandshurica roots, bark, branches, leaves, and immature pericarps showed an inhibitory function on the growth of human cancer cell lines (HeLa cervical carcinoma cells and Bel-7402 hepatoma cells) in vitro [3,4] and on implanted murine tumors (H22 hepatoma and S180 solid tumor) in vivo [5,6]. The absence of juglone in the decoction, the anti-tumor activity of the medicated eggs and the ambiguity of the active principles stimulated us to identify the chemical constituents in the decoction of J. mandshurica and those absorbed by the medicated eggs decocted with this plant. High performance liquid chromatography with diode array detector coupled with quadrupole time-of-flight mass/mass spectrometry through electrospray ionization interface (HPLC-DAD-Q-TOF-MS2 ) was applied for multi-constituent identification
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