Abstract
Leea indica (Vitaceae) is a Southeast Asian medicinal plant. In this study, an ethyl acetate fraction of L. indica leaves was studied for its phytoconstituents using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-microTOF-Q-MS/MS) analysis. A total of 31 compounds of different classes, including benzoic acid derivatives, phenolics, flavonoids, catechins, dihydrochalcones, coumarins, megastigmanes, and oxylipins were identified using LC-MS/MS. Among them, six compounds including gallic acid, methyl gallate, (−)-epigallocatechin-3-O-gallate, myricetin-3-O-rhamnoside, quercetin-3-O-rhamnoside, and 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-β-d-glucopyranoside were isolated and identified by NMR analysis. The LC-MS/MS analysis led to the tentative identification of three novel dihydrochalcones namely 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-rutinoside, 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-glucosylpentoside and 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-(3″-O-galloyl)-β-d-glucopyranoside. The structural identification of novel dihydrochalcones was based on the basic skeleton of the isolated dihydrochalcone, 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-β-d-glucopyranoside and characteristic LC-MS/MS fragmentation patterns. This is the first comprehensive analysis for the identification of compounds from L. indica using LC-MS. A total 24 compounds including three new dihydrochalcones were identified for the first time from the genus Leea.
Highlights
Leea indica (Burm. f.) Merr. (Vitaceae), commonly known as Bandicoot berry, is an evergreen perennial shrub or a small tree of 2 to 16 m in height
(18), [19] and 40,60 -dihydroxy-4-methoxydihydrochalcone 20 -O-β-D-glucopyranoside (27) [20] by comparing their analytical data (1 H, 13 C and 2D-NMR, and LC-MS) with those reported in the literature [16,17,18,19,20]
This study presents the comprehensive identification of chemical constituents of an ethyl acetate fraction of L. indica leaves using HPLC-ESI-microTOF-Q-MS/MS analysis
Summary
Leea indica (Burm. f.) Merr. (Vitaceae), commonly known as Bandicoot berry, is an evergreen perennial shrub or a small tree of 2 to 16 m in height. (Vitaceae), commonly known as Bandicoot berry, is an evergreen perennial shrub or a small tree of 2 to 16 m in height. It is distributed throughout Bangladesh, China, India, Malaysia, Singapore, North Australia, Thailand, and Vietnam [1,2,3]. L. indica is reported to possess various pharmacological activities, e.g., analgesic, anti-angiogenesis, anti-oxidant, anti-inflammatory, anti-microbial, anti-proliferative, hepatoprotective, sedative, and anxiolytic activities [3,5,6,7,8,9,10,11,12]. There are very few reports available on the phytochemistry of L. indica
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