Abstract
Pheromone-binding proteins (PBPs) play a significant role in olfaction and mating. The present work was designed to isolate, extract, and purify the pheromone-binding proteins from the antennae of male Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae). The pheromone-binding proteins extracted from the male antennae were found to be 770 μg in 100 mg of sample. Pheromone-binding protein molecular weight was determined as 17 kDa by SDS-PAGE analysis. Identified proteins were purified through gel extraction with a recovery percentage of proteins up to 95%. Purified protein samples are analyzed on native PAGE gels. Relative mobility of proteins was determined as 0.574 nm in the densitometry analysis. These identified pheromone-binding proteins can be used for identification of novel pheromone compounds in in vitro studies. This study can be helpful in designing integrated pest management programs to control the maize stem borer by mass trapping of male moths.
Highlights
Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) is a devastative pest, majorly hampering the production of several cereal crops, viz., maize, sorghum, sugarcane, and rice
Molecular weight of Pheromone-binding proteins (PBPs) was of 17 kDa and found to be the same in our results using a protein marker (NEX-GEN blue spray pre-stain protein ladder) (Fig. 1)
Extraction and purification of proteins from SDS-PAGE The identified proteins were further purified through gel extraction and recovery of protein varied from 80 to 95%
Summary
Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) is a devastative pest, majorly hampering the production of several cereal crops, viz., maize, sorghum, sugarcane, and rice. OBPs are small, water-soluble, extracellular proteins that are located in the fluid surrounding the sensory dendrite and abundantly found in lymph of insect antennae and other sensory organs (De Biasio et al 2015). They are reflected to be vital in insect olfactory perception at various levels but the exact mechanism is still unknown. Pedda Kasim et al Egyptian Journal of Biological Pest Control (2018) 28:6 the behavior of the receiving individual within the same species (Wyatt 2010) They act as carriers for antagonists and scavengers for pheromone components in female insects (Zhou et al 2009).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
