Identification of phenolic constituents inLonicera caeruleaL. by HPLC with diode array detection electrospray ionisation tandem mass spectrometry

Mayya P Razgonova,Kirill S Golokhvast,Natalia M Mikhailova,Nadezhda G Tikhonova,Svetlana R Luchko,Alexander M Zakharenko,Andrey S Sabitov,Konstantin S Pikula,A.Y Aidiev,E.A Pyanikova,Y Brinza,O.K Nikulina,A.I Hrabovets,A.A Yevglevsky,B.U Baykhozhaeva,L.I Belyaeva,A.A Muratov,V.I Turusov

doi:10.1051/bioconf/20213202010

Abstract

The purpose of this work was a comparative metabolomic study of extracts of Blueberried honeysuckleLonicera caeruleaL.: №1043-11 (St. Petersburg); №1043-08 (St. Petersburg) №863; (Japan); №860 (WildLonicerafrom Amur river) from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources. To identify target analytes in extracts HPLC was used in combination with a BRUKER DALTONIKS ion trap. The results showed the presence of 82 target analytes corresponding to familyCaprifoliaceae. In addition to the reported metabolites, a number of metabolites were newly annotated inLonicera caeruleaL.There were flavonols: Dihydrokaempferol, Rhamnetin I, Rhamnetin II, Taxifolin-3O-glucoside, Mearnsetin-hexoside, Horridin; flavones: Chrysoeriol, Apigenin-O-pentoside, Chrysoeriol-7-Oglucoside; flavanone Naringenin; flavan-3-ols: Catechin, Epicatechin, Biochanin A-7-O-glucoside; essential amino acids: L-Pyroglutamic acid, Tyrosine; polypeptide 5-Oxo-L-propyl-L-isoleucine; sterols: Ergosterol, Fucosterol, Beta-Sitosterin; triterpenoids: Betunolic acid, Oleanoic acid; anabolic steroid Vebonol, indole sesquiterpene alkaloid Sespendole; iridoids: Monotropein,p-Coumaroyl monotropein,p-Coumaroyl monotropein hexoside; Myristoleic acid, etc.

Highlights

Blue-berried honeysuckle Lonicera caerulea L., familyCaprifoliaceae is known as a natural source of food, beverages and nutraceuticals due to its rich chemical composition, enriched with nutrient and biologically active compounds
Phenolic compounds provide antioxidant activity through direct reduction of reactive oxygen species (ROS), inhibition of enzymes involved in oxidative stress, binding of metal ions responsible for ROS production, and stimulation of endogenous antioxidant defense systems [Hossain et al, 2016]
HPLC analysis was done with a ESI detector at wavelengths of 230 ηm and 330 ηm; the temperature corresponded to 17°C

Summary

Introduction

Caprifoliaceae is known as a natural source of food, beverages and nutraceuticals due to its rich chemical composition, enriched with nutrient and biologically active compounds. The increased focus on these berries is due to their phenolic composition, antioxidant activity, and potential health benefits. Phenolic compounds provide antioxidant activity through direct reduction of reactive oxygen species (ROS), inhibition of enzymes involved in oxidative stress, binding of metal ions. The quality and quantity of phenolic compounds in plants usually depends on the stage of growth, the parts of the plant used and the growing conditions in the environment [Bujor O.-C., 2016]. In this regard, the purpose of this work is the simultaneous assessment of phenolic compounds in the berries of Lonicera caerulea L. of various species collected in different climaticgeographical zones of Russia.

Liquid chromatography

Mass spectrometry

Materials

Results and discussion

Chemicals and Reagents

Conclusions