Abstract
The purpose of this work is to study of phenolic compounds in the dry extract of dandelion herb and large burdock leaf tea.Materials and methods . The separation and identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea by thin-layer chromatography and high-performance liquid chromatography with UV-detectionhas been carried out.Results . As a result of research, it has been established that during TLC the optimal system for the separation of phenolic compounds is the ethyl acetate – formic acid – water system (10:2:3). On the chromatogram four spots were found corresponding to the value of Rf and fluorescence in UV-light to flavonoids of the flavone group and phenolic acids (chlorogenic and caffeic acids). For further identification of phenolic compounds using HPLC, eight peaks were found, which in terms of retention time and spectral characteristics correspond to phenologlycosides, chlorogenic acid, caffeic acid derivatives, ferulic acid, umbelliferone.Conclusions . Thus, the dry extract of dandelion herb and large burdock leaf tea contains hydroxycinnamic acids and their derivatives, compounds of coumarin nature, phenologlycosides.
Highlights
Ключевые слова: фенольные соединения, тонкослойная хроматография, высокоэффективная жидкостная хроматография, экстракт сбора одуванчика лекарственного травы и лопуха большого листа сухой
The purpose of this work is to study of phenolic compounds in the dry extract of dandelion herb and large burdock leaf tea
On the chromatogram four spots were found corresponding to the value of Rf and fluorescence in UV-light to flavonoids of the flavone group and phenolic acids
Summary
Цель исследования – изучение фенольных соединений сухого экстракта сбора одуванчика лекарственного травы и лопуха большого листа. Установлено, что экстракт сбора одуванчика лекарственного травы и лопуха большого листа сухой содержит гидроксикоричные кислоты и их производные, соединения кумариновой природы, фенологликозиды. Ключевые слова: фенольные соединения, тонкослойная хроматография, высокоэффективная жидкостная хроматография, экстракт сбора одуванчика лекарственного травы и лопуха большого листа сухой. IDENTIFICATION OF PHENOLIC COMPOUNDS OF DRY EXTRACT OF DANDELION HERB AND LARGE BURDOCK LEAF TEA. The purpose of this work is to study of phenolic compounds in the dry extract of dandelion herb and large burdock leaf tea. The separation and identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea by thin-layer chromatography and high-performance liquid chromatography with UV-detectionhas been carried out.
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