Abstract
In this study, we extracted Aquilaria crassna with aqueous ethanol and water and analyzed the extracts via liquid chromatography diode array detection and electrospray ionization mass spectrometry (LC– ESI–MS) methods. Phenolics were separated using semi-micro HPLC and were identified as iriflophenone 3,5-C-β-diglucoside (1), iriflophenone 3-C-β-glucoside (2), mangiferin (3), iriflophenone 2-O-α-rhamnoside (4), genkwanin 5-O-β-primeveroside (5), genkwanin 5-O-β-glucoside (6), genkwanin 4′-methyl ether 5-O-β-primeveroside (7), and genkwanin (8) via a comparison with authentic samples. The collision-induced dissociation (CID)-MS/MS spectra of these polyphenols and the unknown chromatographic peaks were detected using hybrid ion trap time-of-flight (IT-TOF) mass spectrometry. The results of the present study demonstrated that LC–ESI–MS can be useful for the specific quality control of extracts of extracted A. crassna.
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