Identification of Pepper CaSBP08 Gene in Defense Response Against Phytophthora capsici Infection.

Huai-Xia Zhang,Zhen-Hui Gong,Xiao-Hui Feng,Jing-Hao Jin,Ai-Min Wei,Muhammad Ali,Abdul Mateen Khattak

doi:10.3389/fpls.2020.00183

Abstract

Little information is available on the role of Squamosa promoter binding protein (SBP)-box genes in pepper plants. This family of genes is known to have transcription characteristics specific to plants and to regulate plant growth, development, stress responses, and signal transduction. To investigate their specific effects in pepper (Capsicum annuum), we screened pepper SBP-box family genes (CaSBP genes) for Phytophthora capsici (P. capsici) resistance genes using virus-induced gene silencing. CaSBP08, CaSBP11, CaSBP12, and CaSBP13, which are associated with plant defense responses against P. capsici, were obtained from among fifteen identified CaSBP genes. The function of CaSBP08 was identified in pepper defense response against P. capsici infection in particular. CaSBP08 protein was localized to the nucleus. Silencing of CaSBP08 enhanced resistance to P. capsici infection. Following P. capsici inoculation, the malondialdehyde content, peroxidase activity, and disease index percentage of the CaSBP08-silenced plants decreased compared to the control. Additionally, the expression levels of other defense-related genes, especially those of CaBPR1 and CaSAR8.2, were more strongly induced in CaSBP08-silenced plants than in the control. However, CaSBP08 overexpression in Nicotiana benthamiana enhanced susceptibility to P. capsici infection. This work provides a foundation for the further research on the role of CaSBP genes in plant defense responses against P. capsici infection.

Highlights

Pepper (Capsicum annuum L.) is a valuable solanaceous crop species with high economic as well as high nutritive value worldwide
Pepper Plant CaSBP08, CaSBP11, CaSBP12, and CaSBP13 Genes Are Involved in Resistance to P. capsici
We screened four genes (CaSBP08, CaSBP11, CaSBP12, and CaSBP13) out of the fifteen identified CaSBP genes, each of which responded to P. capsici infection

Summary

Introduction

Pepper (Capsicum annuum L.) is a valuable solanaceous crop species with high economic as well as high nutritive value worldwide. Plants have developed a range of defense mechanisms including physiological, biochemical, molecular, and cellular processes and barriers along with inducible innate immunity (Mou et al, 2017; Hou et al, 2018). Transcription factors have important functions in combating the impact of pathogens through activating or repressing the expression of defense genes (Xu et al, 2011). APETALA2/Ethylene Response Factor-domain transcription factor (AP2/ERF-ORA59) directly regulates the expression of PDF1.2 (PLANT DEFENSIN1.2) in the process of necrotrophic pathogen infection in Arabidopsis (Zarei et al, 2011). JAMYC2 and JAMYC10 are two other MYC transcription factors that are jasmonic acid (JA)-regulated and involved in defense responses against pathogen infection through inducing PDF1.2 gene expression in tomato (Boter et al, 2004; Lorenzo and Solano, 2005)

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