Abstract
Auxin-responsive genes AUX/IAA are important during plant growth and development, but there are few relevant reports in peanut. In this study, 44 AhIAA genes were identified from cultivated peanut, of which 31 genes were expressed in seed at varying degrees. AhIAA-3A, AhIAA-16A and AhIAA-15B were up-regulated, while AhIAA-11A, AhIAA-5B and AhIAA-14B were down-regulated with seed development and maturation. The expression patterns of seven genes, AhIAA-1A, AhIAA-4A, AhIAA-10A, AhIAA-20A, AhIAA-1B, AhIAA-4B and AhIAA-19B, were consistent with the change trend of auxin, and expression in late-maturing variety LM was significantly higher than that in early-maturing EM. Furthermore, allelic polymorphism analysis of AhIAA-1A and AhIAA-1B, which were specifically expressed in seeds, showed that three SNP loci in 3′UTR of AhIAA-1A could effectively distinguish the EM- and LM- type germplasm, providing a basis for breeding markers development. Our results offered a comprehensive understanding of Aux/IAA genes in peanut and provided valuable clues for further investigation of the auxin signal transduction pathway and auxin regulation mechanism in peanut.
Highlights
As one of the important plant hormones, auxin can participate in all aspects of plant growth and development, such as embryo development, apical dominance, organogenesis and morphogenesis, cell division and tissue differentiation, tropism, fruit ripening and organs aging, etc., and be involved in the response to various abiotic stresses, such as salt and drought stress and exogenous hormone treatment [1,2,3,4,5,6,7]
The hypothetical Auxin/indole3-acetic acid (Aux/IAA) protein sequences obtained by the Hidden Markov Model (HMM) analysis in peanut genome were submitted to websites NCBI-CDD, Pfam and SMART to confirm the Aux/IAA domain
44 peanut AhIAA genes were identified by bioinformatics methods, of which 31 genes were expressed in seed at varying degrees
Summary
As one of the important plant hormones, auxin can participate in all aspects of plant growth and development, such as embryo development, apical dominance, organogenesis and morphogenesis, cell division and tissue differentiation, tropism, fruit ripening and organs aging, etc., and be involved in the response to various abiotic stresses, such as salt and drought stress and exogenous hormone treatment [1,2,3,4,5,6,7]. The primary auxin-responsive genes mainly include Auxin/indole3-acetic acid (Aux/IAA), Small Auxin-up RNA (SAUR) and Gretchen Hagen 3 (GH3), which are always represented by a large multigene family in plants [5,7,9,10]. Aux/IAA is in relatively more research studies so far, and it is a gene family with expression specificity in different tissues and developmental stages [7,11]. Studies on Aux/IAA genes and their function analysis have been reported in many other plants, such as cucumber and tomato, as well as in crops including wheat, rice and maize [1,3,11,14,15,16]. With the development and wide application of molecular genetics technology, we are moving towards understanding the clear signal transduction pathway and feedback regulation mechanism of plant auxin
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
