Abstract
Clonal growth of erythroid cells from bone marrow of 2-day-old chicks in fibrin clots under different culture conditions has been used as independent assays for heme-, colony- and burst-stimulating activities found in anemic chicken plasma. The properties of the heme-stimulating activity analyzed by gel filtration, isoelectrofocusing, resistance to heat denaturation, ammonium sulfate precipitation, DEAE-chromatography or HAP-chromatography, suggested serum transferrin as the heme-stimulating factor(s). Heme-, colony- and burst-stimulating factor(s) from anemic chicken plasma did not separate from each other by gel filtration or isoelectrofocusing. Ovotransferrin from egg white also showed heme-, colony- and burst-stimulating activities by the assay employed even after further purification by limited trypsin digestion, electrophoresis, hydroxylapatite chromatography or fractionation by ConA-Sepharose chromatography.
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