Abstract
Extracellular vesicles (EVs) can mediate intercellular communication, including signaling between the placenta and maternal tissues. Human placental explant culture is a versatile in vitro model system to investigate placental function. We performed systematic studies to identify a defined serum-free medium that supports high trophoblast viability and metabolism, as well as release of similar populations of EVs, compared to traditional undefined conditions that contain media additives potentially contaminated with exogenous EVs. We also determined the timeframe in which trophoblast viability and functionality remained optimal. Multiplexed vesicle flow cytometry with classical EV and placenta-specific markers revealed three separate populations, small CD63+ EVs, large PLAP+ EVs, and CD63-/PLAP- EVs. The CD63+ population was higher in the first trimester and the PLAP+ population in the third trimester explants. These culture and analytical approaches will enable in vitro modeling of short-term effects of environmental perturbations associated with pregnancy complications on placental function and EV secretion.
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