Identification of novel target proteins of cGMP/cAMP signaling pathway using cGMP/cAMP capture compound mass spectrometry (CCMS)

Abstract

Background The cyclic nucleotide monophosphates cGMP and cAMP play an essential role in many signaling pathways. The identification and profiling of cGMPand cAMP-binding proteins is an important step in order to elucidate the molecular basis of these pathways. In order to develop a tool to specifically target cGMPand cAMP-binding proteins, we synthesized cGMPand cAMP Capture CompoundsTM (CCs). CCs are trifunctional small molecule probes that carry a small molecule as the selectivity function attached to a scaffold that in addition contains a photo-activatable reactivity function and biotin as a sorting function. We here explored the capability of the cAMPand cGMP Capture Compound Mass Spectrometry (CCMS) approach to display the proteome subset of cGMP-and cAMP-binding proteins.