Abstract
This study was conducted to investigate the growth hormone ( GH; somatotropin-like) gene polymorphisms in 150 water buffalo ( Bubalus bubalis ) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3’ UTR regions of the GH gene (also called GH/Alu I locus) and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene (also called GH/Msp I locus) were amplified, and their PCR products analyzed via DNA sequencing method. Seven [...]
Highlights
The water buffalo or domestic water buffalo originated in India, Southeast Asia, and China
This study was conducted to investigate the growth hormone (GH; somatotropin-like) gene polymorphisms in 150 water buffalo (Bubalus bubalis) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3’ UTR regions of the GH gene and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene were amplified, and their PCR products analyzed via DNA sequencing method
Seven genotypes due to twenty single nucleotide polymorphisms (SNP) and one deletion/insertion were identified in a 347 bp long region of the GH/MspI locus
Summary
The water buffalo or domestic water buffalo originated in India, Southeast Asia, and China. The two classes of domestic water buffalo are swamp buffalo (Bubalus carabanesis) and river buffalo (Bubalus bubalis). While the river buffalo has 50 chromosomes, the swamp buffalo has 48 (Mishra et al, 2015). Anatolian water buffalo reared in Turkey generally have a dark coat color and originated from the Mediterranean subgroup of river buffalo (Soysal, 2013; Konca and Akyüz, 2017). Water buffaloes have been reported to be of great importance to the lives of farmers and to the economies of many countries worldwide (Hussain et al, 2017). Buffaloes have played an important role in the rural
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