Abstract
An RFX-binding site is shown to be conserved in the promoters of a subset of ciliary genes and a subsequent screen for this site in two Drosophila species identified novel RFX target genes that are involved in sensory ciliogenesis.
Highlights
Regulatory factor X (RFX) transcription factors play a key role in ciliary assembly in nematode, Drosophila and mouse
Homologs of C. elegans DAF-19 target genes are regulated by dRFX in Drosophila Our previous work has shown that RFX transcription factors share a common function in ciliogenesis in worm and fly http://genomebiology.com/2007/8/9/R195
Among more than 20 previously identified DAF-19 targets expressed in all ciliated sensory neurons of C. elegans [21,36,37,38], we show that a majority of the homologous genes in fly are down regulated in dRfx mutants (Table 1)
Summary
Regulatory factor X (RFX) transcription factors play a key role in ciliary assembly in nematode, Drosophila and mouse. The proteomic analysis of detergent-extracted ciliary axonemes from cultured human epithelial cells identified 214 proteins [10]. A biochemical fractionation of Chlamydomonas reinhardtii flagella led to the identification of about 700 proteins, of which 360 had high confidence of truly being involved in flagellar composition [11]. Two remarkable approaches took advantage of the availability of complete genome sequences to identify genes encoding ciliary and flagellar proteins. Stolc et al [15] used microarray hybridization to analyze induction levels of all C. reinhardtii genes after deflagellation. They identified 220 genes that are induced at least two-fold and, are likely to be involved in the assembly or function of cilia and flagella
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