Identification of Novel QTL for Seedling Root Architectural Traits in the D Genome of Natural and Resynthetic Allohexaploid Wheat

Abstract

Root architectural traits at the seedling stage have been demonstrated to be crucial for the efficient uptake of nutrients and drought tolerance in wheat. To dissect the genetic basis of these traits from the D genome, 182 recombinant inbred lines (RILs) derived from the common wheat TAA10 crossed with resynthesized allohexaploid wheat XX329 possessed similar AABB genomes were used for QTL mapping of five root traits in hydroponic-cultured seedlings, including lateral root number (LRN), seminal root number (SRN), root hair length (RHL), root diameter (RD), and total root volume (TRV). A total of seven QTLs were identified for the five root traits, with six possible novel QTLs for LRN, RHL, RD and TRV, accounting for 4.98–12.17% of phenotypic variation. One QTL (QLrn.qau-5D.2), controlling lateral root number, was fine mapped an approximate 5.0-Mb interval harboring 80 annotated genes, including five auxin-related genes. We further validated that QLrn.qau-5D.2 in NILTAA10 significantly enhanced yield-related traits, such as plant height, spike length, spike compactness, tiller number per plant and grain yield per plant, as comparison with NILXX329. Collectively, these results provide vital insights for fine-mapping QTLs associated with LRN, SRN, RHL, RD and TRV and facilitate the root morphologic designs for enhancing yield performance.