Abstract
BackgroundThe gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection.ResultsThe results revealed that monoclonal antibody (mAb) JE9 recognized 83WDPQEL88 motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J.ConclusionsA novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J.
Highlights
The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization
We identified a conserved linear B-cell epitope recognised by the monoclonal antibody (mAb) JE9 using synthetic peptides, and applied it for the diagnosis of avian leukosis virus subgroup J (ALV-J) infection using an epitope-based peptide Enzyme-linked immunosorbent assay (ELISA)
We identified WDPQEL as the target sequence of mAb JE9, which corresponds to 83–88 aa of ALV-gp85 as deletion of 83 W or 88 L disrupted the binding of the peptides with mAb JE9
Summary
The gp is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. We mapped the epitope in ALV-J gp by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. Since the first report of avian leukosis virus subgroup J (ALV-J) in the United Kingdom in 1988, the virus has spread rapidly to many countries including China [1,2,3,4,5]. We showed that the gp85-specific mAb JE9 could react with different ALV-J strains but not with other ALV subgroups [13], confirming that the mAb JE9 recognized a conserved antigenic epitope. We identified a conserved linear B-cell epitope recognised by the mAb JE9 using synthetic peptides, and applied it for the diagnosis of ALV-J infection using an epitope-based peptide ELISA. The results in this study will contribute to the understanding of the antigenic structure of gp and rational design of vaccines and diagnostic tools
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